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Microsporidia PCR detection artifacts
due to non-specific binding of the universal microsporidia primers to the rDNA of Arthropod Hosts
Autori:
TOKAREV IURIE, SITNICOVA NATALIA, PISTONE DARIO, LIZHI LUO, SHAOZHE HUANG, IGNATIEVA ANASTASYA, SENDERSKIY IGOR, TODERAŞ ION, MOVILĂ ALEXANDRU, FROLOV ANDREIRezumat:
Within the last decade, PCR has recommended itself as a powerful and reliable tool for numerous applications in biological studies, including cost-effective, rapid and sensitive detection of parasitic microorganisms in body or tissue samples of hosts [7, 10, 11]. Ribosomal DNA is often used as a target for the specific primer binding in the PCR cycle as it includes both conservative and variable regions (allowing performance of various tasks) and is presented by multiple copies within any eukaryotic genome (providing a higher chance for visible amplicon production as compared to single copy genes), and microsporidia are not the exception [1, 2].Domenii:
BiologieNumărul:
1 (310)Anul:
2010Descarcă
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